The lab occupies ~1,100 square feet of contiguous space divided into a wet lab, a cell culture suite, and a small enclosed room for our more sensitive equipment. We have a wide array of standard lab ware, including a refrigerator, freezers, liquid nitrogen tank, equipment for gel electrophoresis, hotplates, balances, water baths, heating blocks, microcentrifuges, rocking platforms, vortexers, a convection oven, a pH meter, and a sonicating bath. The cell culture suite includes two BSL-2 hoods, two incubators, a bench-top centrifuge, an inverted brightfield microscope, a dry bead bath, a Countess automated cell counter, and a live-view microscope for colony picking. The main lab space also contains a ProFlex thermocycler, a Nanodrop 2000c, a Qubit 3.0 fluorometer, a MaxQ heated orbital shaker, custom peristaltic pumps, a Guava easyCyte 8HT flow cytometer (shared with John Wilson), and a Leica DMi8 epifluorescent microscope equipped with a motorized Z-focus scanning stage, a 1.4 MP CCD digital camera, a humidified stage-top incubator to enable long-term culture under normoxia or hypoxia, and 3D deconvolution software for pseudo-confocal capabilities. Finally, we use a 50 L carboy, extra-large magnetic stirring platform, and osmometer to prepare our own custom media for stem cell culture.