Protocols for ipsc-derived brain endothelial cells
As a graduate student, Dr. Lippmann helped pioneer the first protocols for differentiating human induced pluripotent stem cells (iPSCs) to endothelial-like cells with blood-brain barrier (BBB) properties, including expression of appropriate endothelial/BBB markers, active molecular transport systems, and elevated passive barrier function as measured by transendothelial electrical resistance (TEER) and small molecule permeability. Our lab has put forth a substantial amount of effort to make these differentiation protocols more user-friendly and streamlined, particularly by developing fully defined media compositions. We and many others now use these strategies to study the BBB in health and disease.
Relevant contacts
Lexi Yates, Daniel Chavarria
Relevant publications