Protocols for ipsc-derived brain endothelial cells
As a graduate student, Dr. Lippmann helped pioneer the first protocols for differentiating human induced pluripotent stem cells (iPSCs) to endothelial-like cells with blood-brain barrier (BBB) properties, including expression of appropriate endothelial/BBB markers, active molecular transport systems, and elevated passive barrier function as measured by transendothelial electrical resistance (TEER) and small molecule permeability. Our lab has put forth a substantial amount of effort to make these differentiation protocols more user-friendly and streamlined, particularly by developing fully defined media compositions. We and many others now use these strategies to study the BBB in health and disease.
Relevant publications